polyclonal antibody against total egfr sc-03  (Santa Cruz Biotechnology)

Journal: NPJ Precision Oncology

Article Title: Microsecond-timescale MD simulation of EGFR minor mutation predicts the structural flexibility of EGFR kinase core that reflects EGFR inhibitor sensitivity

doi: 10.1038/s41698-021-00170-7

Figure Lengend Snippet: a Growth inhibition curve details. EGFR mutant Ba/F3 cells treated with combination therapy of afatinib and cetuximab. After 72 h of drug treatment, cell viability was measured using the CellTiter-Glo assay ( n = 3). b Growth inhibition curve details. EGFR mutant Ba/F3 cells treated with a combination therapy of EAI-045 and cetuximab. After 72 h of drug treatment, cell viability was measured using the CellTiter-Glo assay ( n = 3). c , d Inhibition of the EGFR signaling pathway in Ba/F3 EGFR mutant cells treated with the combination therapy of afatinib or EAI-045 with cetuximab (10 μg/mL) for 3 h (for afatinib) or 6 h (for EAI-045) was evaluated by western blot with the indicated antibodies.

Article Snippet: Proteins were transferred to polyvinylidene difluoride membranes and immunoblotted with antibodies against phosphor-EGFR (Cell Signaling Technology, #4407 and #3777, 1:1,000), total EGFR (Santa Cruz Biotechnology, sc-03, 1:5,000, or Cell Signaling Technology, #4267), phospho-Akt (Ser473; Cell Signaling Technology, #4060, 1:1,000), total Akt (Cell Signaling Technology, #4691, 1:5,000), phospho-ERK (Cell Signaling Technology, #9101, 1:5,000), total ERK1/2 (Cell Signaling Technology, #9102, 1:5,000), phospoh-S6 (Ser240/244, Cell Signaling Technology, #5364, 1:8,000), total S6 (Cell Signaling Technology, #2217, 1:5,000), or β-actin (Sigma-Aldrich, A5228, 1:1,000).

Techniques: Inhibition, Mutagenesis, Glo Assay, Western Blot

Journal: NPJ Precision Oncology

Article Title: Microsecond-timescale MD simulation of EGFR minor mutation predicts the structural flexibility of EGFR kinase core that reflects EGFR inhibitor sensitivity

doi: 10.1038/s41698-021-00170-7

Figure Lengend Snippet: a Growth inhibition curve details. EGFR mutant expressing Ba/F3 cells treated with serially diluted LY2409881. After 72 h of drug treatment, cell viability was measured using the CellTiter-Glo assay ( n = 3). b Chemical structure of LY2409881. c Inhibition of EGFR phosphorylation in the Ba/F3 cells expressing EGFR-L747P or EGFR-L858R mutation treated with 600 nM of LY2409881 for 3 h was evaluated by western blot with the indicated antibodies.

Article Snippet: Proteins were transferred to polyvinylidene difluoride membranes and immunoblotted with antibodies against phosphor-EGFR (Cell Signaling Technology, #4407 and #3777, 1:1,000), total EGFR (Santa Cruz Biotechnology, sc-03, 1:5,000, or Cell Signaling Technology, #4267), phospho-Akt (Ser473; Cell Signaling Technology, #4060, 1:1,000), total Akt (Cell Signaling Technology, #4691, 1:5,000), phospho-ERK (Cell Signaling Technology, #9101, 1:5,000), total ERK1/2 (Cell Signaling Technology, #9102, 1:5,000), phospoh-S6 (Ser240/244, Cell Signaling Technology, #5364, 1:8,000), total S6 (Cell Signaling Technology, #2217, 1:5,000), or β-actin (Sigma-Aldrich, A5228, 1:1,000).

Techniques: Inhibition, Mutagenesis, Expressing, Glo Assay, Phospho-proteomics, Western Blot